Introduction:
The PepFection mRNATransfection Reagent is a high efficiency mRNA transfection reagent,derived from Biodeg adable non-toxic material and specifically designed for mRNA.This reagent can provide the robust transfection efficiency in a variety of commonly used and hard-to-transfect mammalian cells. such as adherent and suspension cells, as well as primary cells..
Advantages:
❖ Robust transfection efficiency and low cytotoxicity.
❖ Excellent for mRNA transfection.
❖ Compatible with serum and antibiotics in culture medium.
Table 1: Product Package & Storage
|
Cat #
|
Product Name
|
Volume
|
Storage
|
|
T014
|
PepFection mRNATransfection Reagent
|
1 mL
|
2 ~ 8 °C,stable for up to 6 months
-20°C ,stable for up to 12 months
|
|
T014X
|
100mL
|
Important Guidelines for Transfection:
(1) For maximum transfection efficiency, using serum-free medium (such as Opti-MEM® Reduced Serum Medium) to dilute mRNA and the PepFection mRNATransfection Reagent is a must.
(2) While the standard protocols for mRNA transfection being given below, optimization is often needed for maximal transfection efficiency.
(3) It is unnecessary to wash cells and change medium after transfection.