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Biotin-14-dCTP

产品货号：N122

产品规格：50 nmol

产品价格：￥4,000.00

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Product Description:

Biotin-14-dCTP is a dCTP analog which contains biotin attached at the 6 -position of the purine base

by a 14-atom spacer arm. The biotin - labeled nucleotide is incorporated into DNA by nick translation

in the presence of the deoxynucleotide triphosphates dTTP,dGTP and dATP. Other labeling

procedures (i.e., homopolymer, tailing with a terminal deoxynucleotidyl transferase, replacement

synthesis with T4 DNA polymerase or random primed synthesis) may be satisfactory. The

biotin-labeled DNA can be detected colorimetrically using Steptavidin-Alkaline Phosphatase

Conjugate and NBT/BCIP or by chemiluminescence, using streptavidin alkaline phosphatase and an

appropriate chemiluminescent substrate.

References:

1. Vora et al. (2008) Comparison of detection and signal amplification methods for DNA microarrays.

Mol Cell Probes. 22:294-300.

2. Flickinger et al. (1992) Differential incorporation of biotinylated nucleotides by terminal

deoxynucleotidyl transferase. Nucleic Acids Res. 20:2382-2382.

3. Gebeyehu et al. (1987) Novel biotinylated nucleotide–analogs for labeling and colorimetric

detection of DNA. Nucleic Acids Res. 15:4513-4534.

4. Mackey et al. (1993) Use of random primer extension for concurrent amplification and

nonradioactive labeling of nucleic acids. Anal Biochem. Focus® 14, 21.