The RobustDye™ ssDNA Assay Kit provides an easy and accurate
quantitation for ssDNA or oligonucleotides. The kit is not selective for ssDNA
over dsDNA or RNA, but it will not detect contaminating protein or
nucleotides. The assay kit is highly reliable in detecting ssDNA ranging from
50 pg/µL to 200 ng/µL, and offers advantages in stability, linear dynamic
range, and sensitivity over other traditional of DNA quantitation. The kit
contains concentrated assay reagent, dilution buffer, and pre-diluted ssDNA
standards. The assay is performed at room temperature. Simply dilute the
reagent using the buffer provided, add your sample (any volume between 1
µL and 20 µL is acceptable), and read the fluorescence using fluorescence
plate reader or Fluorometer such as Qubit®. The kit is well tolerated to
common contaminants such as proteins, salts, solvents and detergents.
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