The RobustDye™ RNA HS Assay Kit provides a simple, sensitive, and
accurate quantitation for RNA. It contains concentrated assay reagent,
dilution buffer, and pre-diluted RNA standards. The assay kit is highly
selective for RNA, and highly reliable in detecting RNA ranging from 250
pg/µL to 100 ng/µL, and offers advantages in stability, linear dynamic range,
and sensitivity over other traditional of RNA quantitation. The assay is
performed at room temperature, and the signal is stable for 3 hours. Simply
dilute the reagent using the buffer provided, add your sample (any volume
between 1 µL and 50 µL is acceptable), and read the fluorescence using
fluorescence plate reader or Fluorometer such as Qubit® Fluorometer. The
kit is well tolerated to common contaminants such as free nucleotides, salts,
solvents, proteins and detergents (as mentioned in Table 2).
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