The RobustDye™ RNA BR Assay Kit provides a simple, sensitive, and
accurate quantitation for RNA. The kit contains concentrated assay reagent,
dilution buffer, and pre-diluted RNA standards. The assay kit is highly
selective for RNA over dsDNA, and highly reliable for initial sample
concentrations from 1 ng/µL to 1000 ng/µL, and offers advantages in stability,
linear dynamic range, and sensitivity over other traditional methods for RNA
quantitation. The assay is performed at room temperature, and the signal is
stable for 3 hours. Simply dilute the reagent using the buffer provided, add
your sample (any volume between 1 µL and 50 µL is acceptable), and read
the fluorescence using fluorescence plate reader or Fluorometer such as
Qubit® Fluorometer. The kit is well tolerated to common contaminants such
as proteins, free nucleotides, salts, solvents and detergents (Table 2).
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