The RobustDye™ miRNA Assay Kit provides an easy and accurate
quantitation for small RNA (~20 nucleotides or base pairs). The kit is highly
selective for small RNA over rRNA or large mRNA (>1,000 nt). The assay kit
is highly reliable in detecting small RNA ranging from 25 pg/µL to 150 ng/µL,
and offers advantages in stability, linear dynamic range, and sensitivity over
other traditional of small RNA quantitation. The kit contains concentrated
assay reagent, dilution buffer, and pre-diluted miRNA standards. The assay
is performed at room temperature. Simply dilute the reagent using the buffer
provided, add your sample (any volume between 1 µL and 20 µL is
acceptable), and read the fluorescence using fluorescence plate reader or
Fluorometer such as Qubit®. The kit is also well tolerated to common
contaminants such as proteins, salts, solvents and detergents.
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